RNeasy PowerPlant Kit (50)

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Publication protocol

Gene expression analyses: Total RNA was isolated either from Arabidopsis seeds using the RNeasy PowerPlant Kit or from seedlings using RNeasy Plant Mini Kit (QIAGEN) according to the manufacturer’s instructions. DNA in RNA samples was removed with DNase I (Thermo Fisher Scientific) and RNA was reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen). Quantitative PCR was per- formed in 96-well blocks with Brilliant II QPCR Master Mix with ROX (Agilent, #600806) on the AriaMx Real-Time PCR system. Gene expression was normalized using internal control GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C SUBUNIT (GAPC) (At3g04120) [56]. RT-PCR was performed with Taq DNA Polymerase (NEB, #M0273) on a thermal cycler. Analysis of EXPA9 were subjected to amplication for 26 and 30 cycles, and analysis of GAPC was followed by 26 cycles.

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Manufacturer protocol

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