TransMessenger Transfection Reagent (0.5 ml)
siRNA / RNAi /miRNA transfection Mouse - Primary cortical and hippocampal cell
Publication protocol
Transfections of siRNA for endogenous gene targeting were carried out with TransMessenger transfection reagent (Qiagen, Chatsworth, CA). siRNA (1 g per well) was condensed with Enhanser R and formulated with 4 l of TransMessenger reagent, according to the manufacturer’s instructions. The trans- fection complex was diluted in 900 l of Neurobasal and was added directly to the cells; it was replaced with Neurobasal after 2 h. Cells were stained and analyzed 48–68 h after transfections. The results were confirmed in three independent experiments.Full paper Login or join for free to view the full paper.
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