miRNeasy Serum/Plasma Advanced Kit (50)
RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat
Publication protocol
RNA extraction: RNA was extracted from either fresh or frozen plasma according to the manufacturer’s instructions using 6 commercially available kits; Kit A: miRNeasy Serum/Plasma (Qiagen, Germany), Kit B: miRNeasy Serum/Plasma Advanced (Qiagen, Germany), Kit C: Quick-cfRNA Serum & Plasma (Zymo Research, USA), Kit D: Isolate II miRNA (Bioline, Australia), Kit E: PureLink RNA Mini kit (Invitrogen, USA), Kit F: Monarch Total RNA Miniprep kit (New England BioLabs, USA). All extractions were performed in duplicate for each animal, except for the PureLink and Monarch kits due to sample and reagent availability. Following extraction and elution of RNA, samples were immediately frozen at −80 °C.Kit A: miRNeasy Serum/Plasma kit
Briefly, Total RNA was isolated from 200 µl of plasma as per the manufacturer’s recommendations and eluted in 14 µl of RNase-free water.
Kit B: miRNeasy Serum/Plasma Advanced kit
The miRNeasy Serum/Plasma Advanced kit is a phenol-free alternative to the original miRNeasy Serum/Plasma kit. Total RNA was extracted from 200 µl of plasma according to the manufacturer’s protocol and eluted in 20 µl of RNase-free water.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Qiagen for miRNeasy Serum/Plasma Advanced Kit (50) below.
Download manufacturer protocol
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