RNAprotect Bacteria Reagent

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Publication protocol

RNeasy mini kit and phenol-free total RNA purification kit: The cell pellet was reconstituted with 400 µl of sterile minimal media. Then, 800 µl RNAprotect Bacteria Reagent (Qiagen, USA) was added and the contents in the tube were vortexed prior to a 5 min incubation at room temperature (RT). The tubes were centrifuged at 5000×g for 10 min. The supernatant was discarded and the cell pellet was subjected to total RNA extraction and on-column DNase I treatment according to the manufacturers’ protocol.

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Manufacturer protocol

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