QIAamp UltraSens Virus Kit (250)

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Publication protocol

Preparation of samples and controls: Two ml of supernatant from freeze–thawed cell cultures, which corresponds to approximately 5 × 105 cells, was processed with the QIAmp UltraSens virus kit (Qiagen, Valencia, CA, USA) and viral RNA/DNA eluted in 50 μl of H2O. Ten μl of the extracted RNA was reverse-transcribed using the SuperScript III Platinum RT kit (Invitrogen, Carlsbad, CA, USA) and random hexameres according to the manufacturer’s protocol. As positive controls, an enteric adenovirus isolate from stool (type 41) and parainfluenza virus types 1 (ATCC VR-94) and 3 (ATCC VR-93) were used. cDNA from hMPV was kindly provided by Dr. Barbara Huck, Freiburg, Germany

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Manufacturer protocol

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