exoRNeasy Midi Kit (50)
Purification of extracellular vesicles Exosomes - Plasma
Publication protocol
Plasma Exosome Isolation: For each sample, two plasma fractions of 500 µL each were independently filtered with a 0.22 µm filter (Spin-X Centrifuge Tube Filter, Corning Incorporated, Corning, NY, USA) and then used for exosome isolation by a MISEV 2018 [28] approved protocol using the exoRNeasy Midi kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions, which allows the obtention of an exosome enriched fraction by membrane affinity columns. One of the exosome enriched fractions was used for exosome characterization, and the other for RNA extraction.Full paper Login or join for free to view the full paper.
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