exoEasy Maxi Kit (20)

Protocol tips

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

The serum exosome was extracted by exoEasy Maxi Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol with minor modifications. Briefly, serum filtration was used to remove diameters greater than 0.8 µm particles, an equal volume of buffer XBP was added mixed well immediately, and transfered into the exoEasy spin column and centrifuged 1 min at 500 × g, the flow-through was discarded; 10 ml Buffer XWP was added and centrifuged 5 min at 5,000 × g, the flow-through was discarded; the spin column was transfered to a new collection tube, 100 uL Buffer XE was added and centrifuged 5 min at 5,000 × g, the flow-through was collected as exosome resuspension B, named exo-B.

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