EpiTect Fast DNA Bisulfite Kit (200)

Protocol tips

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	Stop the bisulfite reaction with 50 μl NaOH (5 M)

Protocol tips
Stop the bisulfite reaction with 50 μl NaOH (5 M)

Publication protocol

Bisulfite Conversion: Bisulfite conversion and subsequent purification was performed according to the respective kit protocols. The following kits were applied: EpiTect Bisulfite Kit (Catalogue No. 59104), EpiTect Fast DNA Bisulfite Kit (Catalogue No. 59824), EpiTect Fast FFPE Bisulfite Kit (Catalogue No. 59844, Qiagen GmbH, Hilden, Germany), EZ DNA Methylation-Direct Kit (Catalogue No. D5020), EZ DNA Methylation-Gold Kit (Catalogue No. D5005), EZ DNA Methylation-Lightning Kit (Catalogue No. D5030, Zymo Research, Inc., Irvine, CA, USA), innuCONVERT Bisulfite Basic Kit (Catalogue No. 845-IC-1000040), innuCONVERT Bisulfite All-In-One Kit (Catalogue No. 845-IC-2000040), innuCONVERT Bisulfite Body Fluids Kit (Catalogue No. 845-IC-3000040, Analytik Jena AG, Jena, Germany).
For the analysis of inappropriate conversion 1 μmol T5 MeCT5 oligonucleotides were applied to each bisulfite reaction. The bisulfite reactions were stopped with 50 μl NaOH (5 M) without subsequent purification.

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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Fast DNA Bisulfite Kit (200) below.

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