EpiTect Fast Bisulfite Kit (10)

Protocol tips

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	Follow manufacturer's instructions

Protocol tips
Follow manufacturer's instructions

Publication protocol

The DNA obtained from exfoliated cells was bisulfite con- verted using the EZ DNA Methylation kit (Zymo Research, CA, USA, abbreviated as EZ Std) using 200 ng of DNA and following the manufacturer's instructions.
The FFPE material was bisulfite converted using four dif- ferent kits: the EZ Std, the EZ DNA Methylation-Lightning kit (Zymo Research, CA, USA, abbreviated as EZ Lightning), the Epitect Bisulfite kit (Qiagen, Hilden, Germany, abbre- viated as Epitect Std), and the Epitect Fast Bisulfite Kit (Qiagen, Hilden, Germany, abbreviated as Epitect Fast). All kits were used following the manufacturer's instructions with an input of 200 ng in 40 μl of DNA for EZ Std, Epitect Std, and Epitect Fast and 200 ng in 20 μl (maximum rec- ommended volume) for EZ Lightning. Differences between these kits are highlighted in Supp. Table S1.

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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Fast Bisulfite Kit (10) below.

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