EpiTect Fast 96 Bisulfite Kit

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Publication protocol

Reduced Representation Bisulfite Sequencing (RRBS): RRBS was performed as described previously [8, 9]. Briefly, genomic DNA was extracted from iPSC-NPCs (D28) and digested with MspI. Fragments were end-repaired, Illumina adaptor sequences were ligated, and the libraries were bisulfite converted using Qiagen’s EpiTect Fast 96 Bisulfite Kit. Sequencing was performed on Illumina’s HiSeq 2000 using 50 bp single-end reads. Only CpG sites with coverage greater than 5× were included in the analyses.

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Manufacturer protocol

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