miScript II RT Kit (50)

Protocol tips

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

As a preliminary test, microRNA expression was analysed in two samples of each group on custom‐made microRNA arrays (384‐well plate, QIAGEN) containing 183 different mature canine microRNAs, four expression controls (SNORD 61, SNORD 68, SNORD 95 and RNU6‐6P), one reverse transcription control and one PCR control for each sample. Total RNA (100 ng) was reverse transcribed using the miScript II RT Kit (QIAGEN). The cDNA was pre‐amplified using the miScript PreAmp PCR Kit (QIAGEN) according to the manufacturer's instructions on a Mastercycler (eppendorf AG, Hamburg, Germany) with a miScript PreAmp universal primer and a custom miScript PreAmp primer mix. The pre‐amplified cDNA was diluted with 100 μL RNase‐free water.

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Manufacturer protocol

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