Wizard® Genomic DNA Purification Kit

Protocol tips

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	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

Cell Compatibility of Photopolymerization Process
To verify the cell compatibility of the BioInk polymerization process, a cyclobutane pyrimidine dimer (CPD) enzyme-linked immunosorbent assay (ELISA) was performed. CPD formation is one of the predominant types of DNA damage caused by UV light. Dermis models were printed, and genomic DNA was isolated immediately after printing (day 0) and after 5 days of culture with the Wizard Genomic DNA Purification Kit (Promega, Fitchburg, WI) according to the manufacturer’s protocol. Each DNA sample (n = 3) was obtained from the processing of three different and pooled dermal models. Fibroblast DNA content was measured at 260 nm with a NanoDrop (Fisher Scientific, Hampton, NH). As blanks, 3D models were produced by replacing cell suspension with sterile water. In parallel, genomic DNA was isolated from fibroblasts cultured in 2D with and without UV exposure for 10 min at an intensity of 5 mW/cm2 (λ = 260 nm) to obtain positive and negative controls, respectively. The CPD detection was performed with the OxiSelect UV-Induced DNA Damage ELISA Kit (Cell Biolabs, San Diego, CA) following the manufacturer’s guidelines. The sample absorption was measured at 485 nm with a microplate reader (FLUOstar OPTIMA; BMG Labtech, Ortenberg, Germany). The blank absorption values were subtracted from the cell samples’ absorption values.

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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download manufacturer protocol

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