CometAssay Single Cell Gel Electrophoresis Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
1 × 10^5 cells/ml were embedded in LM Agarose (1:10)	The slide was treated with lysis solution, alkali, and subjected to alkaline electrophoresis.

Upstream tips
1 × 10^5 cells/ml were embedded in LM Agarose (1:10)
Protocol tips
The slide was treated with lysis solution, alkali, and subjected to alkaline electrophoresis.

Publication protocol

CometAssay® kit (Trevigen; Gaithersburg, MD) was used for detection of DNA damage, according to the manufacturer's instructions. Briefly, the dissected mice forebrains were immersed into 2 ml of ice‐cold PBS containing 20 mM EDTA, minced into small pieces by using scissors, and homogenized into single cells. The cells obtained were resuspended at a density of 1 × 105 cells/ml, embedded in LM Agarose (1:10), and placed on the CometSlideTM. The slide was treated with lysis solution, alkali, and subjected to alkaline electrophoresis. Finally, samples were stained with intercalating dye and visualized by fluorescence microscopy.

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