DeadEnd™ Colorimetric TUNEL System

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Seed 5x10^3 cells/well in 24-well plates

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Seed 5x10^3 cells/well in 24-well plates

Publication protocol

To examine cellular apoptosis induced by BCH treatment, the TUNEL assay was performed. The cells were seeded at a concentration of 5x10^3 cells/well in 24-well plates. After 24 h growth, the cells were treated with 20 mM BCH at various incubation times. The TUNEL assay was performed using a DeadEndTM Colorimetric TUNEL System kit (Promega Co., Madison, WI, U.S.A.) in accordance with the manufacturer’s instructions.

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Manufacturer protocol

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