CometAssay Single Cell Gel Electrophoresis Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 2x10^5 cells/ml		Quantization of the DNA in the tails of the comets can be performed with Quantity One software (Bio-Rad Laboratories) and ImageJ software version 1.49 (http://rsbweb.nih.gov/ij/index.html).

Upstream tips
Seed 2x10^5 cells/ml
Downstream tips
Quantization of the DNA in the tails of the comets can be performed with Quantity One software (Bio-Rad Laboratories) and ImageJ software version 1.49 (http://rsbweb.nih.gov/ij/index.html).

Publication protocol

For determination of DNA single- and double-strand breaks, a single-cell gel electrophoresis assay was used (Comet Assay TM, Trevigen) per the manufacturer’s instructions after 48-h treatment with trabectedin (0.125 nM) and olaparib (1.25 μM), as single agents or in combination. Quantization of the DNA in the tails of the comets was performed with Quantity One software (Bio-Rad Laboratories) and ImageJ software version 1.49 (http://rsbweb.nih.gov/ij/index.html).

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Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay below.

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