Bioanalyzer High Sensitivity DNA Analysis

DNA Damage Assay SJSA-1

Experiment
DNA Damage Assay SJSA-1
Product
Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Upstream tips
Seed 1.5 X 10^5 cells/ml

Publication protocol

Total DNA was extracted from cultured cells (at least 1.5 × 10^5 cells) and from frozen tissues using the Maxwell® RSC Blood DNA Kit AS1400 (Promega), from FFPE archival tumor biopsy and surgical excision, after microdissection of the tumor area, using the Maxwell® RSC DNA FFPE Kit AS1450 (Promega), and the RSC Maxwell Instrument (Promega) in accordance with the manufacturer’s instructions. DNA purity was checked by NanoDropTM (Thermo Fisher Scientific, Life Technology Italia, Monza, Italy). DNA concentration was determined by the Qubit dsDNA HS (high sensitivity) assay kit (Thermo Fisher Scientific, Life Technology Italia) and the Qubit® 3.0 Fluorometer (Thermo Fisher Scientific, Life Technology Italia). DNA fragmentation was assessed by gel electrophoresis and a 2100 Bioanalyzer High Sensitivity DNA assay Kit (Agilent Technologies, Agilent Technologies, Inc., Santa Clara, CA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA Damage Assay SJSA-1 using Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Agilent Technologies for Bioanalyzer High Sensitivity DNA Analysis below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA Damage Assay SJSA-1 using Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms