Wizard® Genomic DNA Purification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

E. faecium isolate E1002 was grown overnight anaerobically at 37°C in brain heart infusion (BHI) broth (Difco). Genomic DNA was extracted using the Wizard genomic DNA purification kit (Promega) as per manufacturer’s instructions. Genomic DNA sequencing of E. faecium isolate E1002 was then performed using Pacific Biosciences sequencing technology. Briefly, a library was constructed using the PacBio library kit and the size was targeted to 10 kb. Two single-molecule real-time (SMRT) cells were run using P6/C4 chemistry and 240 min video time. A total of 168 614 reads were assembled using the HGAP3 pipeline (Pacific Biosciences). A complete genome of 2 614 649 bp was obtained with a mean coverage of 478×.

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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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