Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram positive Enterococcus faecium

Experiment
DNA isolation / purification Bacteria - Gram positive Enterococcus faecium
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

E. faecium isolate E1002 was grown overnight anaerobically at 37°C in brain heart infusion (BHI) broth (Difco). Genomic DNA was extracted using the Wizard genomic DNA purification kit (Promega) as per manufacturer’s instructions. Genomic DNA sequencing of E. faecium isolate E1002 was then performed using Pacific Biosciences sequencing technology. Briefly, a library was constructed using the PacBio library kit and the size was targeted to 10 kb. Two single-molecule real-time (SMRT) cells were run using P6/C4 chemistry and 240 min video time. A total of 168 614 reads were assembled using the HGAP3 pipeline (Pacific Biosciences). A complete genome of 2 614 649 bp was obtained with a mean coverage of 478×.

Full paper   Login or join for free to view the full paper.

Reviews

Wizard® Genomic DNA Purification Kit from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Enterococcus faecium using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
Complete Genome Sequence of Commensal Isolate E1002
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram positive Enterococcus faecium using Wizard® Genomic DNA Purification Kit from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms