HT 8-oxo-dG ELISA Kit II

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Grow 1-5 x 10^6 adherent cells in complete medium in a suitable tissue culture dish or flask until 75% confluent.

Upstream tips
Grow 1-5 x 10^6 adherent cells in complete medium in a suitable tissue culture dish or flask until 75% confluent.

Publication protocol

A plate-based immunoassay was used to detect the concentration of 8-oxo-dG, a marker of oxidative DNA damage (HT 8-oxo-dG ELISA Kit, R&D Systems, Minneapolis, MN, USA), and The Comet Assay Single Cell Gel Electrophoresis Assay (CN 4250-050-K; R&D Systems, Minneapolis, MN, USA) was used to measure DNA strand breaks following manufacturers’ instructions.

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Manufacturer protocol

Download the product protocol from R&D Systems for HT 8-oxo-dG ELISA Kit II below.

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