Bioanalyzer High Sensitivity DNA Analysis

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1.5 × 10^5 cells/ml/well

Upstream tips
Seed 1.5 × 10^5 cells/ml/well

Publication protocol

Total DNA was extracted from cultured cells (at least 1.5 × 10^5 cells) and from frozen tissues using the Maxwell® RSC Blood DNA Kit AS1400 (Promega), from FFPE archival tumor biopsy and surgical excision, after microdissection of the tumor area, using the Maxwell® RSC DNA FFPE Kit AS1450 (Promega), and the RSC Maxwell Instrument (Promega) in accordance with the manufacturer’s instructions. DNA purity was checked by NanoDropTM (Thermo Fisher Scientific, Life Technology Italia, Monza, Italy). DNA concentration was determined by the Qubit dsDNA HS (high sensitivity) assay kit (Thermo Fisher Scientific, Life Technology Italia) and the Qubit® 3.0 Fluorometer (Thermo Fisher Scientific, Life Technology Italia). DNA fragmentation was assessed by gel electrophoresis and a 2100 Bioanalyzer High Sensitivity DNA assay Kit (Agilent Technologies, Agilent Technologies, Inc., Santa Clara, CA, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA Damage Assay A2780 using Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies.

Manufacturer protocol

Download the product protocol from Agilent Technologies for Bioanalyzer High Sensitivity DNA Analysis below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA Damage Assay A2780 using Bioanalyzer High Sensitivity DNA Analysis from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.