DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram positive Enterococcus faecium

Experiment
DNA isolation / purification Bacteria - Gram positive Enterococcus faecium
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction
Strains were cultivated overnight in brain heart infusion broth at 37 °C. DNA used for PCR or library preparation for Illumina sequencing was extracted by utilizing the DNeasy Blood and Tissue kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). DNA subjected to sequencing by means of Pacific Bioscience SMRT technology was extracted by using the Genomic-tip 100/G kit (Qiagen, Hilden, Germany).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Enterococcus faecium using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
Population structure and acquisition of the resistance determinant in German clinical isolates of ST192
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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