CometAssay Single Cell Gel Electrophoresis Assay

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Seed 1×10^5cells/mL

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Seed 1×10^5cells/mL

Publication protocol

DNA breakage was evaluated using a comet assay kit (Trevigen, Gaithersburg, MD, USA). Three independently reproduced experiments were performed. To determine DNA strand breakage,U-87 cells and HF were plated at 1×10^5cells/ml in a 24-well plate and left to attach for 24 h. The next day, cells were treated with Ln HS (0.2, 2.5, 5, 25, 50μg/mL) for 24 h. Comet slides were stained with diluted SYBR green and examined under an automated robotic epifluorescence microscope with an excitation filter (510 to 550 nm). One hundred cells were analyzed per slide. Experiments were performed in duplicate. Untreated cells served as negative controls

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