CometAssay Single Cell Gel Electrophoresis Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 1–2 × 10^4 cells/ml

Upstream tips
Seed 1–2 × 10^4 cells/ml

Publication protocol

A plate-based immunoassay was used to detect the concentration of 8-oxo-dG, a marker of oxidative DNA damage (HT 8-oxo-dG ELISA Kit, R&D Systems, Minneapolis, MN, USA), and The Comet Assay Single Cell Gel Electrophoresis Assay (CN 4250-050-K; R&D Systems, Minneapolis, MN, USA) was used to measure DNA strand breaks following manufacturers’ instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA Damage Assay MIA PaCa-2 using CometAssay Single Cell Gel Electrophoresis Assay from Bio-Techne.

Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA Damage Assay MIA PaCa-2 using CometAssay Single Cell Gel Electrophoresis Assay from Bio-Techne. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.