DNeasy UltraClean Microbial Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Enterococcal strains were cultured overnight in BHI broth prior to genomic DNA (gDNA) extraction. Extraction was performed using a Qiagen blood and tissue DNeasy kit using a previously reported protocol (38). To isolate E. coli gDNA, bacteria were grown overnight in LB prior to extraction using either the blood and tissue DNeasy kit (Qiagen) or the UltraClean microbial DNA isolation kit (Qiagen) according to the manufacturer’s instructions. Whole-genome amplification (WGA) control DNA was generated by amplification of native gDNA using the REPLI-g kit (Qiagen) according to the manufacturer’s instructions.

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Discussion

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy UltraClean Microbial Kit below.

Download manufacturer protocol

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