CometAssay Single Cell Gel Electrophoresis Assay-Silver

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Suspend cell pellet at 3 x 10^5 cells/mL in 10% (v/v) DMSO, 40% (v/v) medium, 50% (v/v) fetal bovine serum.		Use the OPENCOMET plugin for calculating the tail moment of a comet.

Upstream tips
Suspend cell pellet at 3 x 10^5 cells/mL in 10% (v/v) DMSO, 40% (v/v) medium, 50% (v/v) fetal bovine serum.
Downstream tips
Use the OPENCOMET plugin for calculating the tail moment of a comet.

Publication protocol

Comet assays were performed using CometAssay® Trevigen Kit (4250-050-K) following the manufacturer’s instructions. Pictures were taken using a Nikon Eclipse Ni-E epifluorescence microscope and the tail moment was calculated using the OPENCOMET plugin for Fiji.

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Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay-Silver below.

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