DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Experiment
DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

2.1. Gene cloning
Genomic DNA of S. sviceus ATCC 29083 was isolated from a 0.5 ml DSM 924 bacterial cell culture using the DNeasy Blood & Tissue Kit (Qiagen). The gene encoding the acetyltransferase (ZP_05014886), termed SsAT, was amplified using the polymerase chain reaction (PCR) from S. sviceus genomic DNA. The forward primer had the sequence 5′-CCAGG­GACCAGCAATGTACGGGGCACGCGACCG-3′ and the reverse primer had the sequence 5′-GAGGAGAAGGCG­CGTTATCACCGGCCTTCTTCTGCCGTG-3′. The amplification product was cleaned using a QIAquick Gel Extraction Kit (Qiagen) and was cloned following a ligation-independent cloning (LIC) method (Bonsor et al., 2006 ▶; Fogg & Wilkinson, 2008 ▶) into the vector pETYSBLIC-3C, which incorporates a 3C protease-cleavable N-terminal hexahistidine tag

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
Three-dimensional structure of a GNAT acetyltransferase with similarity to the C-terminal domain of the human GH84 -GlcNAcase
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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