CometAssay Single Cell Gel Electrophoresis Assay-Silver

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Suspend cell pellet at 3 x 10^5 cells/mL in 10% (v/v) DMSO, 40% (v/v) medium, 50% (v/v) fetal bovine serum.	Protocol for alkaline comet assay	Use the OPENCOMET plugin for calculating the tail moment of a comet

Upstream tips
Suspend cell pellet at 3 x 10^5 cells/mL in 10% (v/v) DMSO, 40% (v/v) medium, 50% (v/v) fetal bovine serum.
Protocol tips
Protocol for alkaline comet assay
Downstream tips
Use the OPENCOMET plugin for calculating the tail moment of a comet

Publication protocol

Alkaline comet assays were performed on mouse MECs engineered with CRISPR/Cas9 (gPlant/gRosa26/gNumb/ or gBrca1) using the CometAssay kit (Trevigen, 4250-050-K) according to the manufacturer’s instructions. ~ 100-300 cells were analyzed in each experiment.

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Manufacturer protocol

Download the product protocol from Bio-Techne for CometAssay Single Cell Gel Electrophoresis Assay-Silver below.

Download manufacturer protocol

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