Comet Assay

DNA Damage Assay A-375

Experiment

DNA Damage Assay A-375

Product

Comet Assay from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

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Protocol tips

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Seed 2X10^5cells/ml

Upstream tips
Seed 2X10^5cells/ml

Publication protocol

The presence of DNA fragmentation was examined by single-cell gel electrophoresis (Comet assay), as previously reported [17]. Hydrogen peroxide (H2O2, 30%w/w, Sigma Aldrich Co, St. Louis,MS, USA), an apoptotic inductor in cancer cell lines, was used as a positive control.

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Manufacturer protocol

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