Comet Assay Kits, 96-Well

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 2x10^5 cells/ml	Protocol for Alkaline Comet Assay	Select fifty random cells and analyze the tail moment using OpenComet (open-source automated software)

Upstream tips
Seed 2x10^5 cells/ml
Protocol tips
Protocol for Alkaline Comet Assay
Downstream tips
Select fifty random cells and analyze the tail moment using OpenComet (open-source automated software)

Publication protocol

To assess SSB, COV362, cells were exposed to NE at the time points required for each experiment: 1 h or 24 h. After NE exposure, the cells were collected and processed to perform the alkaline version of the CometAssay® as described by the manufacturer (Trevigen, Gaithersburg, MD, USA). The nuclei were stained with Yoyo-1® and visualized using the EVOS™ M7000 Imaging System (Thermo Scientific, Rockford, IL, USA). Fifty nuclei were randomly selected and analyzed for the percentage of DNA in tail using the Comet Analysis Software (Trevigen, Gaithersburg, MD, USA). Ethyl methanesulfonate (EMS) (Sigma, St. Louis, MO, USA) was used as a positive control at a concentration of 12 mM for 4 h.

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Manufacturer protocol

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