Comet Assay Kits, 96-Well

DNA Damage Assay SK-OV-3

Experiment
DNA Damage Assay SK-OV-3
Product
Comet Assay Kits, 96-Well from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Upstream tips
Seed 2x10^5 cells/ml
Protocol tips
Protocol for Alkaline Comet Assay
Downstream tips
Select fifty random cells and analyze the tail moment using OpenComet (open-source automated software)

Publication protocol

To assess SSB, SKOV3 cells were exposed to NE at the time points required for each experiment: 1 h or 24 h. After NE exposure, the cells were collected and processed to perform the alkaline version of the CometAssay® as described by the manufacturer (Trevigen, Gaithersburg, MD, USA). The nuclei were stained with Yoyo-1® and visualized using the EVOS™ M7000 Imaging System (Thermo Scientific, Rockford, IL, USA). Fifty nuclei were randomly selected and analyzed for the percentage of DNA in the tail using the Comet Analysis Software (Trevigen, Gaithersburg, MD, USA). Ethyl methanesulfonate (EMS) (Sigma, St. Louis, MO, USA) was used as a positive control at a concentration of 12 mM for 4 h.

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Papers

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Manufacturer protocol

Download the product protocol from Cell Biolabs for Comet Assay Kits, 96-Well below.

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