PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Experiment
DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Product
PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

First experiment. In the first experiment, two different
filtration methods were used for both tap and river water. The first was based on a nitrocellulose filter (Merck, Millipore), 90 mm diameter, in a stainless steel holder (Millipore). For river water, it was necessary to include pre-filters with variable pore size. Subsequently, one quarter of the filter was transferred to a 2 ml tube containing four 3.2 mm chrome steel beads and one millilitre of TE buffer, and homogenised in a MagNALyser (Roche). DNA isolation was performed from 250 µl of the supernatant using the PowerSoil DNA isolation kit (MoBio USA).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen.

Paper title
Comparison of filtering methods, filter processing and DNA extraction kits for detection of mycobacteria in water.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

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Videos

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