PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

First experiment. In the first experiment, two different
filtration methods were used for both tap and river water. The first was based on a nitrocellulose filter (Merck, Millipore), 90 mm diameter, in a stainless steel holder (Millipore). For river water, it was necessary to include pre-filters with variable pore size. Subsequently, one quarter of the filter was transferred to a 2 ml tube containing four 3.2 mm chrome steel beads and one millilitre of TE buffer, and homogenised in a MagNALyser (Roche). DNA isolation was performed from 250 µl of the supernatant using the PowerSoil DNA isolation kit (MoBio USA).

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

Download manufacturer protocol

Videos

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