QIAamp DNA Mini Kit

DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Experiment
DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Mycobacterial DNA extraction.
Two column-based DNA extraction methods (QIAamp DNA minikit [Qiagen, Germany] and QuickGene DNA tissue kit S for QuickGene-Mini80 [QG] [Kurabo, Japan]) were tested, each with and without a pretreatment step using the MolYsis Basic5 kit (Molzym, Germany) to remove human DNA. The samples that were pretreated with the MolYsis kit were processed following the manufacturer's protocol. After the pretreatment step, the manufacturer's protocol for the QIAamp DNA minikit method of DNA purification from blood or body fluids (Spin Protocol) was followed, and a modified DNA tissue kit S manufacturer's protocol for the QG method was followed.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis using QIAamp DNA Mini Kit from Qiagen.

Paper title
Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) Cultures
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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