QIAamp DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Mycobacterial DNA extraction.
Two column-based DNA extraction methods (QIAamp DNA minikit [Qiagen, Germany] and QuickGene DNA tissue kit S for QuickGene-Mini80 [QG] [Kurabo, Japan]) were tested, each with and without a pretreatment step using the MolYsis Basic5 kit (Molzym, Germany) to remove human DNA. The samples that were pretreated with the MolYsis kit were processed following the manufacturer's protocol. After the pretreatment step, the manufacturer's protocol for the QIAamp DNA minikit method of DNA purification from blood or body fluids (Spin Protocol) was followed, and a modified DNA tissue kit S manufacturer's protocol for the QG method was followed.

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download manufacturer protocol

Videos

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