PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Experiment
DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae
Product
PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

MoBio PowerSoil DNA extraction procedure
One 500 mg fecal aliquot was used for MoBio PowerSoil DNA isolation kit extraction. 5 ml of MoBio lysis buffer was immediately added to the fresh fecal sample, which was then vortex mixed for 30–40 seconds. Fecal suspensions were then centrifuged (1,500 g×5 minutes) and 1 ml of the supernatant placed into the MoBio Garnet bead tubes containing 750 ul of MoBio buffer. These tubes were then heated at 65°C for 10 minutes, then at 95°C for 10 minutes. Samples were then stored at −80°C prior to processing in both laboratories following the manufacturer's instructions. DNA was eluted in 100 µL MoBio elution buffer.



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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit from Qiagen.

Paper title
The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing
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Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

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