PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

MoBio PowerSoil DNA extraction procedure
One 500 mg fecal aliquot was used for MoBio PowerSoil DNA isolation kit extraction. 5 ml of MoBio lysis buffer was immediately added to the fresh fecal sample, which was then vortex mixed for 30–40 seconds. Fecal suspensions were then centrifuged (1,500 g×5 minutes) and 1 ml of the supernatant placed into the MoBio Garnet bead tubes containing 750 ul of MoBio buffer. These tubes were then heated at 65°C for 10 minutes, then at 95°C for 10 minutes. Samples were then stored at −80°C prior to processing in both laboratories following the manufacturer's instructions. DNA was eluted in 100 µL MoBio elution buffer.



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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

Download manufacturer protocol

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