QIAamp DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA preparation
We used six different bacterial strains such as: ATCC 29212 and ATCC 29213 as Gram positive and ATCC 19606, ATCC 25922, ATCC 27853 and ATCC 13882 as Gram negative using three different kits: a-Qiamp DNA Mini Kit (Qiagen, Germany), b-Fermentas (Fermentas, Lithuania), and c-Cinagen DNA extraction kit (dNTP, Iran) to evaluate efficiency was applied for DNA extraction of strains maintained in solid media as follows: 200 μl of bacterial suspension (0.5 McFarland) was subjected to individual extraction protocols as indicated by each manufacturer. Qualitative and quantitative assessment of DNA preparation was performed through agarose gel and spectrophotometric measurements.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

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