QIAamp DNA Mini Kit

DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Experiment
DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA preparation
We used six different bacterial strains such as: ATCC 29212 and ATCC 29213 as Gram positive and ATCC 19606, ATCC 25922, ATCC 27853 and ATCC 13882 as Gram negative using three different kits: a-Qiamp DNA Mini Kit (Qiagen, Germany), b-Fermentas (Fermentas, Lithuania), and c-Cinagen DNA extraction kit (dNTP, Iran) to evaluate efficiency was applied for DNA extraction of strains maintained in solid media as follows: 200 μl of bacterial suspension (0.5 McFarland) was subjected to individual extraction protocols as indicated by each manufacturer. Qualitative and quantitative assessment of DNA preparation was performed through agarose gel and spectrophotometric measurements.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae using QIAamp DNA Mini Kit from Qiagen.

Paper title
Development of PCR-based method for detection of in septicemia
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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