Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Protein Ladder Prestained

Experiment

Protein Ladder Prestained

Product

Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028) from Abcam

Manufacturer

Abcam

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
Size range: 10-245 kDa Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).	Do not heat, dilute, or add a reducing agent before loading. Load 1.5 - 2.5µl per well for general Western transferring and 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

Upstream tips
Size range: 10-245 kDa Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
Protocol tips
Do not heat, dilute, or add a reducing agent before loading. Load 1.5 - 2.5µl per well for general Western transferring and 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

Publication protocol

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Reviews

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Papers

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Manufacturer protocol

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Videos

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