MagNA Pure Compact Nucleic Acid Isolation Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Upstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Publication protocol

Staphylococcus epidermidis, Streptococcus mitis, Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger and Fusarium solani were mixed with an internal control virus and treated with: 1) proteinase K; 2) lyticase and 3) lyticase followed by proteinase K. DNAs was manually extracted using the QIAmp DNA Mini kit or the MagNA Pure Compact automate. DNA extraction yields and the inhibitors were assessed with a phocid Herpesvirus. Bacterial detection was performed using TaqMan real-time PCR and yeasts and filamentous Fungi with HRM (real-time PCR followed by high-resolution melting analysis).


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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

Download manufacturer protocol

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