MagNA Pure Compact Nucleic Acid Isolation Kit I

DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Experiment
DNA isolation / purification Bacteria - Gram negative Helicobacter pylori
Product
MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
For isolation of nucleic acids from relatively large organisms
such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Publication protocol

Genomic DNA was extracted from the whole blood samples with EDTA using automated system for DNA isolation Magna Pure Compact Nucleic Acid Isolation Kit I (Roche Applied Science, Germany) on fully automated platform MagNa Pure Compact System in accordance to the instructions by the manufacturer.34 Complete nucleotide sequences of individual genes for inflammatory cytokine IL-1β (rs16944), TNF-α (rs1800629) and TLR-4 (rs4986790) were looked into online databases National Center for Biotechnology Information (NCBI; www.ncbi.nlm.nih.gov) and ENSEMBLE (www.ensembl.org). The sequences were examined with the help of the software package Vector NTI Advance 11 (Invitrogen, Carlsbad, CA, USA).21,25,26 Polymorphisms genotyping was performed using the KASP technology (KBioscience competitive Allele-Specific PCR) using primers and reagents Kasp On Demand (KOD) (KBioscience, UK). 120 bp long reference sequences were sent to the manufacturer, upon which the appropriate primers and probes were designed (Table 1).

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Discussion

Discussion

2 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

2 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Helicobacter pylori using MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences.

Paper title
Comparison of Helicobacter pylori genotypes obtained from the oropharynx and stomach of the same individuals - a pilot study.
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

Download PDF Download manufacturer protocol

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