MagNA Pure Compact Nucleic Acid Isolation Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Upstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Publication protocol

Genomic DNA was extracted from the whole blood samples with EDTA using automated system for DNA isolation Magna Pure Compact Nucleic Acid Isolation Kit I (Roche Applied Science, Germany) on fully automated platform MagNa Pure Compact System in accordance to the instructions by the manufacturer.34 Complete nucleotide sequences of individual genes for inflammatory cytokine IL-1β (rs16944), TNF-α (rs1800629) and TLR-4 (rs4986790) were looked into online databases National Center for Biotechnology Information (NCBI; www.ncbi.nlm.nih.gov) and ENSEMBLE (www.ensembl.org). The sequences were examined with the help of the software package Vector NTI Advance 11 (Invitrogen, Carlsbad, CA, USA).21,25,26 Polymorphisms genotyping was performed using the KASP technology (KBioscience competitive Allele-Specific PCR) using primers and reagents Kasp On Demand (KOD) (KBioscience, UK). 120 bp long reference sequences were sent to the manufacturer, upon which the appropriate primers and probes were designed (Table 1).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Helicobacter pylori using MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram negative Helicobacter pylori using MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.