IEF Marker 3-10, Liquid Mix

Protein Ladder IEF and 2-D Standards

Experiment

Protein Ladder IEF and 2-D Standards

Product

IEF Marker 3-10, Liquid Mix from SERVA Electrophoresis

Manufacturer

SERVA Electrophoresis

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• IEF Marker 3-10 contains nine native proteins in the PI range 3.5 to 10.7 (13 isoforms).	• Staining with SERVA Violet 17 (cat. no. 35072 and 35074), SERVA Blue G (cat. no. 35050), SERVA Blue R (cat. no. 35051): 5 µl/lane for horizontal gels, 20 µl/lane for vertical gels. • Silver staining: 1:10 dilution and 5 µl or 20 µl/lane

Upstream tips
• IEF Marker 3-10 contains nine native proteins in the PI range 3.5 to 10.7 (13 isoforms).
Protocol tips
• Staining with SERVA Violet 17 (cat. no. 35072 and 35074), SERVA Blue G (cat. no. 35050), SERVA Blue R (cat. no. 35051): 5 µl/lane for horizontal gels, 20 µl/lane for vertical gels. • Silver staining: 1:10 dilution and 5 µl or 20 µl/lane

Publication protocol

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Papers

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Manufacturer protocol

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Videos

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