MagNA Pure Compact Nucleic Acid Isolation Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA isolation.
In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) (Table 1 and details below). In a second step, a variety of modifications to two Qiagen kits were examined, namely, the QIAamp DNA stool minikit (QIAStool) and the QIAamp Fast DNA stool minikit (QIAFast). The standard operating procedure for an improved DNA isolation method (i.e., QIAamp Fast DNA stool modified, corresponding to QIAFast+BB.GBT+2Trans described here) can be found at https://dx.doi.org/10.6084/m9.figshare.3475406. For details regarding the individual DNA isolation procedures, see the supplemental materials and methods (see Text S1 in the supplemental material).

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Discussion

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

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