MagNA Pure Compact Nucleic Acid Isolation Kit I

DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment
DNA isolation / purification Bacteria - Gram negative Salmonella enterica
Product
MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA isolation.
In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) (Table 1 and details below). In a second step, a variety of modifications to two Qiagen kits were examined, namely, the QIAamp DNA stool minikit (QIAStool) and the QIAamp Fast DNA stool minikit (QIAFast). The standard operating procedure for an improved DNA isolation method (i.e., QIAamp Fast DNA stool modified, corresponding to QIAFast+BB.GBT+2Trans described here) can be found at https://dx.doi.org/10.6084/m9.figshare.3475406. For details regarding the individual DNA isolation procedures, see the supplemental materials and methods (see Text S1 in the supplemental material).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Salmonella enterica using MagNA Pure Compact Nucleic Acid Isolation Kit I from Roche Lifesciences.

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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact Nucleic Acid Isolation Kit I below.

Download PDF Download manufacturer protocol

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