Macrolide – R/MG ELITe MGB®Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• M. genitalium - 23S rRNA gene; 5 mutations: A2058G, A2059G, A2058T, A2058C, A2059C, associated with macrolide-resistance by melt-curve analysis

Upstream tips
• M. genitalium - 23S rRNA gene; 5 mutations: A2058G, A2059G, A2058T, A2058C, A2059C, associated with macrolide-resistance by melt-curve analysis

Publication protocol

The ELITe InGenius platform (ELITechGroup) is a fully auto-mated sample-to-result PCR system that performs nucleic acid extraction and real-time PCR amplifica-tion directly from primary patient specimens. The Macrolide-R/MG ELITe MGB kit is a CE-IVD-markedassay that uses the ELITe InGenius platform. This assay targets the 23S rRNA gene to detectM. genitaliumandfive mutations associated with macrolide resistance (A2058G, A2058C, A2058T, A2059G, andA2059C) (Table 1). An endogenous human gene is detected as an internal control, and the exogenousCPE internal control is used for urine specimens. The Macrolide-R/MG ELITe MGB kit was validated onurine. Validation is ongoing on cervical and vaginal swabs. Primary specimens (200ml) were loaded ontothe ELITe InGenius platform according to the manufacturer’s instructions, and data were analyzed usingELITe InGenius software version 1.3.0.12

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Manufacturer protocol

Download the product protocol from ELITechGroup for Macrolide – R/MG ELITe MGB®Kit below.

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