Allplex™ MG & AziR Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• CE-IVD-marked multiplex real-time PCR assay that detects M.genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance		• Analyze data using Seegene viewer software version 1.6

Upstream tips
• CE-IVD-marked multiplex real-time PCR assay that detects M.genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance
Downstream tips
• Analyze data using Seegene viewer software version 1.6

Publication protocol

The Allplex MG & AziR assay (Seegene, Republic of Korea) is a CE-IVD-marked multiplex real-time PCR assay that detects M. genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance (A2058G, A2058C, A2058T, A2059G, A2059C, and A2059T [Escherichia coli numbering]) (Table 1). An endogenous human gene is detected as an internal control, except in urine specimens, to which the exogenous ASTI IC(2) internal control is added prior to DNA extraction. The Allplex MG & AziR kit was validated using urine, genital swabs, and liquid-based cytology. This assay was performed using 5ml of DNA extract and a CFX96 real-time PCR system (Bio-Rad) according to the manufacturer's instructions. Data were analyzed using Seegene viewer software version 1.6

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Manufacturer protocol

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