Wizard® Genomic DNA Purification Kit

DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Experiment

DNA isolation / purification Bacteria - Gram negative Salmonella enterica

Product

Wizard® Genomic DNA Purification Kit from Promega

Manufacturer

Promega

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

Genomic DNA extraction
Total DNA was extracted from reference strains with the Wizard Genomic DNA purification Kit (Promega, Wisconsin, United States). For clinical isolates, we increased the throughput of the extraction step with the InstaGene matrix kit (Bio-Rad, Marnes-la-Coquette, France). Both DNA extraction methods were performed according to the manufacturer's instructions. For PCR, we used DNA resuspended in a rehydration buffer provided with the Wizard kit or the final supernatant of the InstaGene matrix extraction process.

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Reviews

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Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

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Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative Salmonella enterica using Wizard® Genomic DNA Purification Kit from Promega.

Paper title

CRISPR Is an Optimal Target for the Design of Specific PCR Assays for Serotypes Typhi and Paratyphi A

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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

Download manufacturer protocol

Videos

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