Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
| • Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlert™ Kit allowing the use of less sensitive plate luminometers or multifunctional readers. |
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| Upstream tips |
| • Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlert™ Kit allowing the use of less sensitive plate luminometers or multifunctional readers. |
Publication protocol
A supernatant from a hybridoma culture (Hybridoma B lymphocyte, R4-6A2 (ATCC number HB-170)) contaminated by mycoplasma was collected and confirmed with the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza, Basel, Switzerland) to be positive. A test with a serial dilution of this medium was performed and verified that there was detectable contamination even to a 1:128 dilution. The medium was sterilized with a 0.22-μM filter to make sure to use a bacteria-free medium culture (mycoplasma are smaller than common bacteria, typically between 0.1–0.8 μm in diameter, so a fair part of them should pass through the mesh). Ten percent of FBS and 2 mM GlutaMax (Gibco by Life Technologies) were added to this filtered medium and a second MycoAlert™ test was performed to check again the positivity
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