MycoAlert® Mycoplasma Detection Kit (50 Tests)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.	• The positive control for the MycoAlert® Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set.

Upstream tips
• They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.
Protocol tips
• The positive control for the MycoAlert® Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set.

Publication protocol

Enzymatic MycoAlert™ Mycoplasma detection kit was used according to the manufacturer’s instructions. Briefly, the ratio of the ATPs level in each sample before (Reading A) and after (Reading B) the addition of MycoAlert™ substrate, was considered an indicator for the presence of Mycoplasma contamination. The presence of contamination was proved if the Reading B/Reading A ratio was greater than 1

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Manufacturer protocol

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