ResistancePlus® MG FleXible

Protocol tips

Upstream tips	Protocol tips	Downstream tips
• The Resistance Plus MG FleXible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of M. genitalium and four 23S rRNA mutations associated with macrolide resistance.	• Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions	• Analyze data using GeneXpert Dx system version 5.1 software

Upstream tips
• The Resistance Plus MG FleXible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of M. genitalium and four 23S rRNA mutations associated with macrolide resistance.
Protocol tips
• Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions
Downstream tips
• Analyze data using GeneXpert Dx system version 5.1 software

Publication protocol

The ResistancePlus MG FleXible kit (SpeeDx, Australia) utilizes single-use FleXible cartridges. The kit was adapted from the Resistance Plus MG kit (SpeeDx) for the the GeneXpert system (Cepheid), a fully automated sample-to-result PCR system that performs nucleic acid extraction and real-time PCR amplification directly from primary patient specimens. The Resistance Plus MG Flexible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of. genitalia and four 23S rRNA mutations associated with macrolide resistance (A2058G, A2058C,A2058T, and A2059G) (Table 1). The kit was validated on urine, vaginal, cervical, urethral, and rectal swabs. Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions. Data were analyzed using GeneXpert Dx system version 5.1 software

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Manufacturer protocol

Download the product protocol from SpeeDx for ResistancePlus® MG FleXible below.

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